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full-length human abhd2  (Thermo Fisher)


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    Structured Review

    Thermo Fisher full-length human abhd2
    Characterisation of <t>ABHD2</t> inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves
    Full Length Human Abhd2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full-length human abhd2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    full-length human abhd2 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "ABHD2 activity is not required for the non-genomic action of progesterone on human sperm"

    Article Title: ABHD2 activity is not required for the non-genomic action of progesterone on human sperm

    Journal: bioRxiv

    doi: 10.1101/2024.12.17.628646

    Characterisation of ABHD2 inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves
    Figure Legend Snippet: Characterisation of ABHD2 inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves

    Techniques Used: Inhibition

    Endogenous steroids (5 µM) do not modulate the enzymatic activity (A) nor lead to thermal stabilization (B) of ABHD2(L22-E425). A. Enzymatic activity. B. DSF curves of representative compounds
    Figure Legend Snippet: Endogenous steroids (5 µM) do not modulate the enzymatic activity (A) nor lead to thermal stabilization (B) of ABHD2(L22-E425). A. Enzymatic activity. B. DSF curves of representative compounds

    Techniques Used: Activity Assay

    Thermostabilization of ABHD2 by inhibitors in intact cells. A. Cells were incubated with compound 1, compound 6 (171) or DMSO followed by a 3 minute incubation at the indicated temperatures. Remaining ABHD2 protein levels were monitored by lumincescence and normalized to cells incubated at 37°C. B. Cells incubated with the indicated compounds, then heated for 3 minutes at 50°C. The remaining ABHD2 was measured and normalized as in A.
    Figure Legend Snippet: Thermostabilization of ABHD2 by inhibitors in intact cells. A. Cells were incubated with compound 1, compound 6 (171) or DMSO followed by a 3 minute incubation at the indicated temperatures. Remaining ABHD2 protein levels were monitored by lumincescence and normalized to cells incubated at 37°C. B. Cells incubated with the indicated compounds, then heated for 3 minutes at 50°C. The remaining ABHD2 was measured and normalized as in A.

    Techniques Used: Incubation



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    full-length human abhd2  (Thermo Fisher)
    90
    Thermo Fisher full-length human abhd2
    Characterisation of <t>ABHD2</t> inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves
    Full Length Human Abhd2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/full-length human abhd2/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    full-length human abhd2 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterisation of ABHD2 inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves

    Journal: bioRxiv

    Article Title: ABHD2 activity is not required for the non-genomic action of progesterone on human sperm

    doi: 10.1101/2024.12.17.628646

    Figure Lengend Snippet: Characterisation of ABHD2 inhibitors. (A) structures of the inhibitors with enzymatic IC50 values and T M shifts obtained with ABHD2(L33-E425). (B) representative inhibition curves. (C) representative melting and derivative curves

    Article Snippet: For expression in insect cells, a codon optimized insert encoding for full-length human ABHD2 was synthesized at Thermofisher Scientific and cloned via Gateway technology (Invitrogen) into pD-INS26, a vector backbone produced in-house by modification of pVL1393 to encode for a C-terminal eGFP-6xHis tag.

    Techniques: Inhibition

    Endogenous steroids (5 µM) do not modulate the enzymatic activity (A) nor lead to thermal stabilization (B) of ABHD2(L22-E425). A. Enzymatic activity. B. DSF curves of representative compounds

    Journal: bioRxiv

    Article Title: ABHD2 activity is not required for the non-genomic action of progesterone on human sperm

    doi: 10.1101/2024.12.17.628646

    Figure Lengend Snippet: Endogenous steroids (5 µM) do not modulate the enzymatic activity (A) nor lead to thermal stabilization (B) of ABHD2(L22-E425). A. Enzymatic activity. B. DSF curves of representative compounds

    Article Snippet: For expression in insect cells, a codon optimized insert encoding for full-length human ABHD2 was synthesized at Thermofisher Scientific and cloned via Gateway technology (Invitrogen) into pD-INS26, a vector backbone produced in-house by modification of pVL1393 to encode for a C-terminal eGFP-6xHis tag.

    Techniques: Activity Assay

    Thermostabilization of ABHD2 by inhibitors in intact cells. A. Cells were incubated with compound 1, compound 6 (171) or DMSO followed by a 3 minute incubation at the indicated temperatures. Remaining ABHD2 protein levels were monitored by lumincescence and normalized to cells incubated at 37°C. B. Cells incubated with the indicated compounds, then heated for 3 minutes at 50°C. The remaining ABHD2 was measured and normalized as in A.

    Journal: bioRxiv

    Article Title: ABHD2 activity is not required for the non-genomic action of progesterone on human sperm

    doi: 10.1101/2024.12.17.628646

    Figure Lengend Snippet: Thermostabilization of ABHD2 by inhibitors in intact cells. A. Cells were incubated with compound 1, compound 6 (171) or DMSO followed by a 3 minute incubation at the indicated temperatures. Remaining ABHD2 protein levels were monitored by lumincescence and normalized to cells incubated at 37°C. B. Cells incubated with the indicated compounds, then heated for 3 minutes at 50°C. The remaining ABHD2 was measured and normalized as in A.

    Article Snippet: For expression in insect cells, a codon optimized insert encoding for full-length human ABHD2 was synthesized at Thermofisher Scientific and cloned via Gateway technology (Invitrogen) into pD-INS26, a vector backbone produced in-house by modification of pVL1393 to encode for a C-terminal eGFP-6xHis tag.

    Techniques: Incubation